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KMID : 1161520140180050318
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Animal Cells and Systems
2014 Volume.18 No. 5 p.318 ~ p.323
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Negative regulation of peroxiredoxin 6 (Prdx 6) transcription by nuclear oncoprotein DEK during leukemia cell differentiation
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Kim Kee-Beom
Chae Yun-Cheol
Han A-Rim
Kang Joo-Young
Jung Hyeon-Soo
Park Jin-Woo
Hahm Ja-Young
Kim Se-Ryeon
Seo Sang-Beom
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Abstract
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The oncogene protein DEK is an abundant and ubiquitous nuclear protein with implications in acute myelogenous leukemia, as translocation which results in the formation of a DEK-CAN fusion protein. In a previous study, we have identified that DEK negatively regulated peroxiredoxin 6 (Prdx 6) transcription synergistically with the p65 subunit of NF-κB. In this study, we further investigated DEK-mediated transcriptional regulation of Prdx 6 during leukemia cell differentiation. Using Chromatin Immunoprecipitation analysis and Prdx 6 reporter assays, we found that DEK operated as a negative regulator of Prdx 6 transcription during leukemia cell differentiation. DEK was highly expressed and recruited to Prdx 6 promoter along with p65 and repressed transcription after leukemia cell differentiation.
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KEYWORD
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DEK, Prdx 6, leukemia, differentiation
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